Litaba - Tlhahiso ea Vanillin ea tlhaho e tsoang lisebelisoa tse ncha tse ncha

I.introdududududududududududududududududududute

Vanillin ke e 'ngoe ea metsoako e tummeng ka ho fetisisa le e tloaelehileng lefatšeng. Ka sebele, e ntšitsoe ho linaoa tsa Vella, tse theko e phahameng ebe li tobana le mathata mabapi le khatello ea kelello le ho phephela chain. Leha ho le joalo, ka tsoelo-pele ho Biotechnology, haholo-holo lebaleng la likokoana-hloko tsa likokoana-hloko, mosebetsi o mocha oa tlhahiso ea vanillin Vanillin e hlahile. Ho sebelisa likokoana-hloko tsa likokoana-hloko tsa mofuta oa tlhaho tsa thepa ea tlhaho ea tlhaho e fane ka tsela e sebetsang ea moruo bakeng sa li-cannthesis tsa vanillina. Mokhoa ona ha o bue feela ka lintlha tse amehang feela empa li fana ka tharollo ea methapo ea lithaba. Patlisiso e Khantšitsoeng ke Setsi sa SMR sa saense le theknoloji (Smis) e fane ka tlhahlobo e felletseng ea li-vanontin tsa likotlo tsa likonyana le lits'ebetsong tsa eona mabapi le khoebo ea lijo.

II. Mokhoa oa ho fumana vanilin ea tlhaho ho tsoa lisebelisoa tse ncha tse ncha

Ts'ebeliso ea Seculic Acid e le substrate

Ferlic acid, e tsoang melumong joalo ka makhopho a litšila le vanillin 'me a sebetsa e le tlhahiso e sebelisetsoang tlhahiso ea Vanillin. Likokoana-hloko tse fapaneng tse kang pseudomonas, aspergillas, li-streptomys, li-fungus tsa li-fungus tse sebelisitsoeng bakeng sa tlhahiso ea Vanillin ho tsoa Fanlico acid. Haholo-holo, mefuta ea kang amycolatopsis le fungi e tšoeu e tsejoa e le bakhethoa bakeng sa ho hlahisa vanillin ho tsoa ho Fileil ho tsoa ho Filelic acid. Ho bile le lipatlisiso tse 'maloa tlhahiso ea Visalin ho tloha bo-'mati ba li-microorgalisms, mekhoa ea enzorgasms, e totobatsang phapang le bokhoni ba mokhoa ona.

Enzzymatic Synthes of Seanzylis oa Vanzymatic ea Forlic acid e kenyelletsa hydroyl esterase, e leng lihlahisoa tsa vanilline le li-ganzy. Ka ho hlahloba boholo ba chelete ea vanillin biosynthetic ho litsamaiso tsa mahala tsa selefouno Ntle le moo, ts'ebeliso ea lisele tsa likokoana-hloko e sentse ka lebaka la setsebi sa eona se setle le botsitso tlas'a maemo a fapaneng. Mokhoa oa ho itlosa Van Jovel bakeng sa tlhahiso ea Vanillin oa ho tima ha Ferlic a atisoa ho etsoa tlhoko ea matloana. Mokhoa ona o kenyelletsa COCKEMEA-Penzylase ea Deczyme le Conzyme e ikemetseng e ikarabellang bakeng sa phetoho ea ho fetohela ho Vanillin. Ho akheha ha cdc le CSO2 ho thusa ho hlahisoa ha vanillin ho tloha bofokoli ba Sefaneng se leshome, tlhahiso ea bitillin e sa sebetseng.

Ts'ebeliso ea Eugenol / Isoeugebonol e le substrate

Eugenol le Isoeulagnol, ha ba kenngoa Sefathine le Metabotoni e amanang le eona, e fumanoeng ea ho ba le lisebelisoa tse fapaneng le boleng ba moruo. Boithuto bo 'maloa bo sebelisitsoe ts'ebeliso ea liphatsa tsa lefutso ebile e etsahala likokoana-hloko tsa tlhaho ho kenya vanilize ho tloha Eugon. Monyetla oa ho senya eugenol o hlokometsoe ho libaktheria tse fapaneng le li-fungus tsa Euge le li-fungus empa li sa fuoe bacillus, li-psedococcccccccccccccccccccccccccccccccccccccccst. Phatlalatso ea Eugenol Oxidase ea Eugenol (Eugo) E le enzyme bakeng sa tlhahiso ea vanillin tikolohong e kholo ea indasteri e bontšitse bokhoni bo boholo tikolohong e kholo. Eugo a bonts'a botsitso le tšebetso ea phallo e pharaletseng, e nang le ts'ebetso e potlakileng ea Eugo e ntseng e eketseha le ho fokotsa nako. Ho feta moo, ts'ebeliso ea eufe e sa sebetseng e lumella ho hlaphoheloa ha biocatalyst ho fihla ho tsekisano tse 18 tsa karabelo ho feta. Ka mokhoa o ts'oanang, enzyme e samake e ka khothaletsa phetoho ea Isoeugnol ho vanillin ntle le ho kena maloetse.

Likarolo tse ling

Ntle le botsitso le Eugenel, metsoako e meng joalo ka vanilic acid le c6-c3 makhopho. VICANIC ACIVID e hlahisitsoeng e le sehlahisoa sa lignrin kapa e le karolo ea metabolic litseleng tsa metabolic, e nkuoa e le sengoliloeng sa se-liehente tse sebelisoang. Ho feta moo, ho fana ka leseli ho tšebeliso ea C6-C3 Phenylpropanis bakeng sa tlhahiso e ikhethang ea tatso e tsitsitseng ea cannnthes.

Qetellong, ts'ebeliso ea lisebelisoa tse ncha tse nchafang bakeng sa tlhahiso ea vanillin ea likoloi tsa vanillin ka li-micbalimation tsa likotoana tsa microbial ke nts'etsopele ea letsopa. Mokhoa ona o fana ka tsela e 'maloa ea ho hlahisoa ea Vanillin, ea tšoaeang lintho tse re amang le ho fokotsa mekhoa ea tloaelo ea mekhoa ea setso. Likopano tse fapaneng le boleng ba moruo ka ho fetela indasteri ea lijo ho hatisa bohlokoa ba ho etsa lipatlisiso le nts'etsopele sebakeng sena. Tsoelo-pele ea nakong e tlang lebaleng la tlhahiso ea Vanillin ea Vanillin e na le monyetla oa ho lokisa indasteri ea tatso, e fana ka mefuta e meng e khotsofatsang bakeng sa tatlo e tsitsitseng ea ho tabolla. Ha re ntse re tsoela pele ho sebelisa matla a lisebelisoa tse ncha tse nchafatsoang le tsoelo-pele ea vanilline ea tlhaho ho tsoa ka har'a subtination e tšepisitsoeng bakeng sa matloana a ts'ehetsoang.

III. Melemo ea ho sebelisa lisebelisoa tse ncha tse nchafatsoang ho hlahisa vanilin ea tlhaho

Tikoloho e botsoalle:U sebelisa lisebelisoa tse ncha tse ncha tse kang limela le litšila tsa tsoekere ho hlahisa chelete ea vanillin, fokotsa litšusumetso tse mpe tikolohong, 'me u fokotsa tlhahiso e mpe ea limela.

Ho Tšelisoa:Ho sebelisa lisebelisoa tse ncha tse thusang ho phepelo ea matla le lisebelisoa tse tala, ho thusa ho sireletsa matlotlo a tlhaho le ho fihlela litlhoko tsa meloko e tlang.

Tšireletso ea BIVEATESTIO:Ka tšebeliso ea lisebelisoa tse ngata tse hatisoang, lisebelisoa tsa limela tse hlaha li ka sireletsoa, tse tlatlapa tšireletsong ea mefuta-futa ea limela le tlhokomelo ea lipalo.

Boleng ba sehlahisoa:Ha ho bapisoa le selekane sa maiketsetso, vanillin ea tlhaho e ka ba le menyetla e mengata ea monko le tlhaho e tla thusa ho ntlafatsa boleng ba lihlahisoa tsa tatso le khatello ea maikutlo.

Fokotsa ho itšepa ho fussil fuels:Ts'ebeliso ea lisebelisoa tse ncha tse nchafatsoang e thusa ho fokotsa ho hlomphuoa ha lithaole, e leng molemo ho ts'ireletso ea matla le matla a matla a matla a ts'ireletso. Kea ts'epa hore tlhaiso-leseling e kaholimo e ka araba lipotso tsa hao. Haeba u hloka tokomane ea litšupiso ka Senyesemane, ke kopa u ntsebise hore nka u fa eona.

IV. Sephetho

Monyetla oa ho sebelisa lisebelisoa tse ncha tse nchafalloang ho hlahisa vanilin e 'ngoe e le mokhoa o tsitsitseng oa le tikolohong o bohlokoa. Mokhoa ona o tšepisa ho sebetsana le tlhoko e ntseng e eketseha ea vanillin ea tlhaho ha u ntse u fokotsa ho itšetleha ka mekhoa ea maiketsetso.

Natunte ea tlhaho ea vanilin e na le boemo ba bohlokoa ba ho phela, ba bohlokoa bakeng sa monko o tšosang ea monko o tšosang ea monko o tšosang ebile o sebelisoa e le moetsi oa liaparo. Ho bohlokoa ho totobatsa bohlokoa ba Sechaba sa tlhaho sa Vanillin ea tlhaho ka mor'a ho bakoala lijo, ho tšoana le maikutlo a bona a tsitsipano bakeng sa litatso tsa tlhaho.

Ntle le moo, tšimo ea Tlhahiso ea Vanillin Vanillin e hlahisa menyetla e matla ea ho etsa lipatlisiso tse ling le nts'etsopele. Hona ho kenyelletsa ho hlahloba mekhoa e mecha ea mahlale le mekhoa e mengata ea ho ntlafatsa katleho le ts'ehetso ea ho hlahisa chelete ea tlhaho ea vanilline ea tlhaho. Ho feta moo, nts'etsopele ea mekhoa e sentsoeng le e theko e tlaase e tla ba karolo ea bohlokoa ea ho amohela sebaka sa vanily e tšoarellang le ea eco-monate indastering ea tatso.